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The Remediation of Copper Ions by Shoot Cultures of Solanum Melongena and Its Influence on The Production of Phytosterols and Phytosteroids

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Title The Remediation of Copper Ions by Shoot Cultures of Solanum Melongena and Its Influence on The Production of Phytosterols and Phytosteroids
 
Creator Kok, Tjie
 
Subject QR Microbiology
 
Description This study aimed to observe the remediation of copper ions by shoot cultures of solanum melongena and its influence on the production of phytosterol(s) and phytosteroid(s). The cultures were able to remove 46.6-66.5% of the ions from media containing 80-320 µM of the ions and accumulated them in their biomass. Application of copper ions concentration higher than that of control Murashige and Skoog media modified with the addition of benzyladenine 4 µg/L caused a decrease in total free sterols content of the cultures. The higher the copper ions concentrations, the higher the decrease in total free sterols content was. Spotting the hydrolysate extracts of the cultures on tin layer chromatography(TLC) plate, developing using a mixture of CHCI3:CH3OH(10:1), and spraying the spots by Dragendorff’s reagent-followed by heating for 5 minutes at 1000C, there was a blue spot with Rf (retardation factor) value similar to that of standard (solasodine). The chromatograms of gas chromatograph (GC) showed that the retention time (rt) of certain substance contained in the extracts is similar to that of solasodine. Verification using mass spectrometer (MS) showed that the ionic fragments of the substance are similar to those of standard solasodine (dilute). It could then be predicted that the shoot cultures of solanun melongena were be able to produce solasodine. Further verification is needed to ensure whether it was solasodine or not; it can be conducted by using nuclear magnetic resonance (NMR)
 
Date 2010-10-17
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier /621/1/Tjie%20Kok_The%20Remediation%20of%20Copper%20Ions_2010.pdf
Kok, Tjie (2010) The Remediation of Copper Ions by Shoot Cultures of Solanum Melongena and Its Influence on The Production of Phytosterols and Phytosteroids. In: 2010 3rd International Conference on Enviromental and Computer Scince, 17-19 Oktober 2010, Kunning, China.