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PRINSIP UMUM DAN PELAKSANAAN POLYMERASE CHAIN REACTION (PCR) [General Principles and Implementation of Polymerase Chain Reaction]

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Title PRINSIP UMUM DAN PELAKSANAAN POLYMERASE CHAIN REACTION (PCR)
[General Principles and Implementation of Polymerase Chain Reaction]
 
Creator Handoyo, Darmo
Rudiretna, Ari
 
Subject QH301 Biology
QH426 Genetics
 
Description Polymerase Chain Reaction (PCR) is an in vitro technique for the amplification of a specific DNA region without prior transfer into living cells. It is a powerful technique because a million-fold amplification can be achieved only in a few hours. For the carrying out of PCR, pair of primers are needed that flank the DNA region to be amplified. A primer is an oligonucleotide with a nucleotide sequence complementary to the nucleotide sequence in
the DNA template. This paper will discuss the general principles of PCR, the detailed procedure for carrying out the PCR and the various factors affecting the optimal PCR results. This technique was introduced by Kary Mullis in 1985, for which he obtained the Nobel Prize in 1993.
 
Publisher University of Surabaya
 
Date 2000-09
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier /35/1/ART002.pdf
Handoyo, Darmo and Rudiretna, Ari (2000) PRINSIP UMUM DAN PELAKSANAAN POLYMERASE CHAIN REACTION (PCR) [General Principles and Implementation of Polymerase Chain Reaction]. Unitas, 9 (1). pp. 17-29. ISSN 0854-3097