Record Details

Reduction of a-tocopherylquinone Model Compound With Various Reductant

Ubaya Repository

View Archive Info
 
 
Field Value
 
Relation http://repository.ubaya.ac.id/33/
 
Title Reduction of a-tocopherylquinone Model Compound With Various Reductant
 
Creator Kohar, Indrajati
 
Subject RS Pharmacy and materia medica
 
Description In order to study the possibility of tranformation of a-tocopherylquinone (TQ) into a more oxidiseable compound and also to find out the recycling effect in the cells, an experiment was conducted by reducing the model compound 2-(3- hydroxy-3-methylbutyl)-3,5,6-trimethyl-1,4-benzoquinone (PQ) with various reductants.
In the experiment it was shown that glutathione did not reduce PQ,nor NADH by itself, so the effective reductant in the NADH/FAD combination must have been FADH2. Thus there is a probability that in a biological system, the
most probable reductant for TQ would be a flavin enzyme rather that ascorbic acid or glutathione. The non-physiological dithiothreitol was as effective as NADH/
FAD which is interesting because of its similarity to the physiologically important reduced lipoic acid.
The reactivity of the various reductants used in this experiment decrease in the order of dithiothreitol ~ NADH/FAD (8/10) > sodium dithionite > NADH/FAD (2:10)
> sodium ascorbate > ascorbic acid (Fig.8).
 
Publisher University of Surabaya
 
Date 2001-03
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier /33/1/Art0001_Indrajati.pdf
Kohar, Indrajati (2001) Reduction of a-tocopherylquinone Model Compound With Various Reductant. Unitas, 9 (2). pp. 3-17. ISSN 0854-3097